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Image Search Results
Journal: JCI Insight
Article Title: 1,25-Dihydroxyvitamin D 3 regulates furin-mediated FGF23 cleavage
doi: 10.1172/jci.insight.168957
Figure Lengend Snippet: Treatment with a furin inhibitor ( A ) reduces cFGF23, ( B ) increases iFGF23, and ( C ) reduces cFGF23/iFGF23 in the plasma of WT and Vdr –/– mice ( n = 6–11 per group). ( D ) The furin inhibitor diminishes serum phosphate in WT mice. ( E ) OCY454 cells with a knockdown of Vdr ( Vdr -KD OCY) exhibit a higher cFGF23/iFGF23 ratio than control cells ( Cas9 OCY), and furin inhibition increases the relative abundance of iFGF23 in both Cas9 OCY and Vdr KD OCY cells. ( F ) Quantification of Western blot bands by ImageJ. ( G ) Vdr KD does not increase cFGF23 in OCY454 with GalnT3 overexpression ( GalnT3 Tg) and Fam20C KD; β-actin blots are derived from the same samples run contemporaneously in parallel gels. ( H ) Quantification of Western blot bands by ImageJ (NIH). Data represent the mean ± SEM. P values by 2-tailed Student’s test. FI, furin inhibitor; Scr, scrambled.
Article Snippet: Membranes were blocked with 5% milk in TBS-Tween and incubated with FGF23 (186-206, 21-6310; Immutopics), VDR (ab109234, Abcam), Furin (sc-133142, Santa Cruz Biotechnology), GALNT3 (PA5-89560, Invitrogen, Thermo Fisher Scientific) or
Techniques: Clinical Proteomics, Knockdown, Control, Inhibition, Western Blot, Over Expression, Derivative Assay